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KMID : 0613320000060040143
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Journal of the Korean Bone and Joint Tumor Soceity
2000 Volume.6 No. 4 p.143 ~ p.151
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p53 Mutations in Ewing’s Sarcoma
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Bae Dae-Kyung
Sun Seung-Deok
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Abstract
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Purpose : The p53 tumor suppressor gene is one of the most frequently altered genes in human malignancies. We try to explore the implication of p53 alteration in Ewing’s sarcoma.
Materials and Methods : We analyzed 35 paraffin blocks to explore the deletion and sequence alterations of p53.
Results : Quantitative PCR analysis showed that 2 tumors showed a homozygous deletion of the gene. Mutational analysis of exons 4 to 9 of p53 by PCR-SSCP revealed that 3 tumors carry sequence alterations in exons 5 or 8, and DNA sequencing analysis identified missense point mutations.
Conclusion : Taken together, our data demonstrate that p53 is genetically altered in a small
fraction of Ewing’s sarcoma.
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KEYWORD
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Ewing’s sarcoma, p53, Mutation
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